Effect of Vitamin E and Selenium Supplementation on Antibody Titer against to Hemorrhagic Septicemia Vaccine in Buffalo Calves
نویسندگان
چکیده
Vitamin E and selenium are considered as immune boosting agents. The present study was designed to evaluate effect of vitamin E and selenium on antibody titer against to hemorrhagic septicemia (HS) vaccine. Twenty unvaccinated buffalo calves (two to four month old) were randomly selected and divided into four groups (group A, B, C and D); and each group contain five calves. Group A was control; Group B was vaccinated against to HS vaccine, Group C was supported with vitamin E and selenium supplementation (VESS) and Group D was vaccinated against to HS along with VESS. Group C and D were supplement with VESS at day 0. After fifteen days, vaccine was administered to Group B and D. Three serum samples were collected at day 0, 30 and 45 of experimental trial from each buffalo calf. Samples were analyzed by complement fixation test to estimate antibody titer. One-way analysis of variance was performed to compare the treatment means and Tuckey’s post hoc test was used to find difference between the treated groups. The level of significance was 0.05. Results showed that significantly higher antibody titer was developed in the group D (73.5±28.62) as compared to antibody titer of Group B (42.2±17.52) at day 45 of experimental trial. No development of antibody titer in Group A and C was observed at any stage. It was concluded that vitamin E and selenium significantly improve the antibody titer when it is given along with HS vaccine in buffalo calves.
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